Development of the zebrafish lateral line request pdf. A framework for understanding morphogenesis and migration. The zebrafish sensory posterior lateral line is an excellent model system to study collective cell migration and organogenesis. Schematic of migration of the pllp from the otic vesicle to the tip of the tail as.
Zebras are just one of the many of herbivores that migrate in search of food every year. This plays an essential role in orientation, predatory behavior, defense, and social schooling. The posterior lateral line in zebrafish has emerged as an excellent system to study how a sensory organ system develops. The lateral line consists of two groups of receptors located on the body surface. To study the molecular basis of neural crest differentiation, we have cloned the gene disrupted in the mont blanc mobm610 mutation, which affects all neural crest derivatives. A description of zebrafish posterior lateral line pll primordium development at single cell resolution together with the dynamics of wnt, fgf, notch and chemokine signaling in this system has allowed us to develop a framework to understand the selforganization of cell fate, morphogenesis and migration during its early development. Polarization and migration in the zebrafish posterior lateral line system article pdf available in plos computational biology 4. Zebrafish lateral line primordium migration leaves behind clusters of cells that will form neuromasts, which are mechanoreceptive organs that allow the fish to detect zebrafish lateral line migration mbl embryology class of 2012 on vimeo.
Lateral line definition of lateral line by medical. Secondary lateral line canals although the secondary lateral line canals branched from main canals were short and simfig. Migration in fishes with diagram biology discussion. We present the current knowledge concerning the formation of the chemokine gradient, its interpretation within the cell, and the molecular mechanisms under. We examined this process in the case of the posterior lateral line pll, a mechanosensory system present in fish and amphibians. A sheath of motile cells supports collective migration of. Until now, printers have not been able to directly print pdfs. Kif1b interacts with kbp to promote axon elongation by. Control of cell migration in the zebrafish lateral line. Sep 17, 20 zebrafish lateral line primordium migration leaves behind clusters of cells that will form neuromasts, which are mechanoreceptive organs that allow the fish to detect water movement. A description of zebrafish posterior lateral line pll primordium development at single cell resolution together with the dynamics of wnt, fgf, notch and. A conspicuous part of the system is a line of organs that extends along each flank and which gave the system its name. Five uniformly, pigmented, horizontal stripes on the side of the body, all extending onto the end of caudal fin rays. Fisheries and aquaculture encyclopedia of life support.
Each and every year, thousands of zebra migrate in search for better grazing pastures. The posterior lateral line pll nerve developed normally in the injected wildtype siblings. Lateral line system in scomberomorus niphonius 41 between the prc and mdc, and dorsal parts of the ioc, prc and stc. Powerful techniques allow efficient generation and recovery of zebrafish mutations. Cell proliferation in the developing lateral line system. A guide for the laboratory use of zebrafish danio rerio. Pattern formation in the lateral line of zebrafish. Phenotypedriven chemical screening in zebrafish for. It is made up of a series of mechanoreceptors called neuromasts lateral. How to describe the function of a lateral line in a fish. The lateral line system comprises a set of sense organs, the neuromasts, arranged under the skin in species. Sylvatica are derived from cells that migrate from. Molecular dissection of the migrating posterior lateral line.
Chemokineguided cell migration and motility in zebrafish. For more information see haas and gilmour 2006 chemokine signaling mediates selforganizing tissue migration in the. In the last decade, highthroughput chemical screening has become the dominant approach for discovering novel compounds with therapeutic properties. Zebrafish or zebra danio danio rerio are seen as one of the latest models for vertebrate embryological development studies.
They begin as grooves that develop in the epidermis along the main lateral lines. The lateral line of fish and amphibians is a sensory system that comprises a number of individual sense organs, the neuromasts, arranged in a defined pattern on the surface of the body. Sea lampreys are so destructive that under some conditions, only one out of seven fish attacked by a sea lamprey will survive. The sensory ability is achieved via modified epithelial cells, known as hair cells, whi.
Cell migration plays an essential role in many morphogenetic processes, and its deregulation has many dramatic consequences. A framework for understanding morphogenesis and migration of the. Neural crest progenitor cells are the main contributors to craniofacial cartilage and connective tissue of the vertebrate head. During embryogenesis, zebra fish cardiac precursor cells cpcs originating from anterior lateral plate mesoderm migrate toward the midline between the endoderm and the yolk syncytial layer ysl to form cardiac tube. Migration in fishes with diagram article shared by. The following points highlight the six main types of migration in fishes.
Special hairs in the pores sense changes in water pressure from the movements of other fish or predators. Zebra migration facts about zebra migratory patterns. The sense organs of the posterior lateral line system neuromasts are formed by a migrating primordium. The largest zebra migration is the annual migration of more than two million from the serengeti to kenya. The zebrafish posterior lateral line primordium pllp, a group of approximately 120 cells which migrates from the otic vesicle to the tip of the tail, spearheading the development of the lateral line sensory system, is an excellent model to study such collective migration in an in vivo context. Zebrafish lateral line primordium migration leaves behind clusters of cells that will form neuromasts, which are mechanoreceptive organs that allow the fish to detect water movement. Here, we investigate the relation between cell migration and cell proliferation in the particular case of the development of the zebrafish lateral line. In other words, the lateral line detects the presence of nearby animals and objects that cause or disrupt water flow coombs and montgomery, 1999. These progenitor cells also give rise to the pigment, neuronal and glial cell lineages.
In the development of most fish, however, a number of structures called lateralline canals are formed as a secondary specialization. Organization and development of the zebrafish posterior lateral line. Kif1b interacts with kbp to promote axon elongation by localizing a microtubule regulator to growth cones. The lateral line is a row of pores running along the fishs sides from head to tail. Pdf ildr1b is essential for semicircular canal development. Cellular and developmental biology, part b developmental biology, the second volume on the topic in the methods in cell biology series, looks at methods for analyzing cellular and developmental biology of zebrafish. The lateral line is a system of sense organs found in aquatic vertebrates, used to detect movement, vibration, and pressure gradients in the surrounding water. The lateralline system is a simple sensory system comprising a number of discrete sense organs, the neuromasts, distributed over the body of fish and amphibians in speciesspecific patterns. Migration can take years and may cover thousands of miles.
Cargos of particular importance for axon outgrowth are microtubule modifiers, such as scg10 stathmin2. The lateral line system is a simple sensory system comprising a number of discrete sense organs, the neuromasts, distributed over the body of fish and amphibians in speciesspecific patterns. Why is the lateral line system important to most fishes. In the zebrafish, these advantages can be combined with a wealth of genetic tools, making this system ideally suited to a combined molecular, cellular and genetic analysis. Based on the extensive body of literature that has been established for analysis of lateral line ll regeneration in embryonic and larval zebrafish 8,24,25, the goal of our study was to develop a quantitative assay for lateral line regeneration in zebrafish that could be applied to disease models that are best studied in the adult fish.
An over view on the causes, types and role of hormones in. Cephalic lateral line system in scomberomorus niphonius mostly based on nsmtp 108635, 63. Its development involves fundamental biological processes such as longrange cell migration, planar cell polarity, regeneration, and postembryonic. Fish use the lateral line sense organ to sense prey and predators, changes in the current and its orientation and they use it to avoid collision in schooling. Shootins mediate collective cell migration and organogenesis of the. Fisheries and aquaculture encyclopedia of life support systems eolss ii 3. The lateral line helps a fish sense or hear movements of other fish it may feed on or other animals that may harm it. During its migration, the primordium deposits clusters of cells at regular intervals, each of which will eventually differentiate as a neuromast. The original description is brief and provides little information to separate this species from a general synodus body plan with the exception of the mention of transverse barring on the trunk, 55 mucous canal scales in the lateral line, a projected snout, and 12 pectoral and analfin rays. The sensory ability is achieved via modified epithelial cells, known as hair cells, which respond to displacement caused by motion and transduce these signals into. Building the posterior lateral line system in zebrafish ncbi.
Periodic deposition of neuromasts by the posterior lateral line primordium pllp a. However, it is now clear that the lateral line is involved as a sensor of water motion, or hydrodynamic stimulation, that arises within a few body lengths of a fish. Sea lampreys have had an enormous negative impact on the great lakes fishery. Fish have internal ears otoliths that are buried beneath the skin on. While the lateral line helps detect the next meal, more senses come into play before the actual dining. Dynamic fgf signaling couples morphogenesis and migration. Feb 24, 2017 the lateral line is a system of sense organs found in aquatic vertebrates, used to detect movement, vibration, and pressure gradients in the surrounding water. Overview zebrafish danio rerio has emerged recently as a premiere organism to study vertebrate development and genetics. Ongoing genetic screens have identified over 7,000 mutations in zebrafish affecting genes that regulate developmental patterning, organogenesis, physiology and behavior. Mitotic patterns in the migrating lateral line cells of. In zebrafish, the pll placode delaminates at 1820 h postfertilization hpf by a still. Building the posterior lateral line system in zebrafish. Abstract in terrestrial and aquatic organisms, migration is a very common phenomenon that occurs both on diel and seasonal bases either for, reproduc.
The lateral line in fish, as characterized in amia calva and atractosteus spatula, is recognized as a series of grooves lateral line canals traversing the surface of the mandible and upper skull. Lateral line definition of lateral line by medical dictionary. In a groundbreaking study, a team of researchers that includes wwf staff discovered africas longest land mammal migration. Morphology of the mechanosensory lateral line system in. The lateral line may also allow fish to swim consistently in schools, changing directions and speeds quickly, without colliding. Overview of lateral line migration in zebrafish youtube. These embryos have the great advantage that they develop as see through embryos, that is, all internal development can be clearly observed from the outside in the living embryo. Zebrafish lateral line primordium migration leaves behind clusters of cells that will form neuromasts, which are mechanoreceptive organs that allow the fish to detect.
The lateral line system is simple comprising six cell types, its sense organs form according to a defined and reproducible pattern, and its neurons are easily visualized. Larval development of the lateral line the company of biologists. According to parker 1904 the first people to recognise the pores of the lateral line were stenon in. Sensory systemsfishlateral line wikibooks, open books for. The embryo is anesthetized, removed from the chorion, and mounted between bridged coverslips westerfield, 1994, for ob servation and later recovery. A lateral line is a sense organ fish use to detect movement and vibration in the surrounding water. It also helps fish stay upright in the water column. Ildr1b is essential for semicircular canal development, migration of the posterior lateral line primordium, and hearing ability in zebrafish. S1ps1p2 signaling in cardiac precursor cells migration. Learn vocabulary, terms, and more with flashcards, games, and other study tools. The migration of plains or burchells zebra stretches from namibia to botswanaa distance of more than 300 miles roundtrip. Role of the lateral line in fish behaviour springerlink.
They use their eyes and something called a lateral line to hold their places in the school. A guide for the laboratory use of zebrafish danio rerio, university of oregon press, eugene, or 1994. The zebrafish lateral line primordium is a migrating epithelial placode whose function is to deposit a series of mechanosensory hair cell organ progenitors, termed proneuromasts, along a stripe of the chemokine sdf1a cxcl12a zebrafish information network that runs along each flank of the embryo ghysen and damblychaudiere, 2007. Lateral line system, a system of tactile sense organs, unique to aquatic vertebrates from cyclostome fishes lampreys and hagfish to amphibians, that serves to detect movements and pressure changes in the surrounding water. Though critical for neural circuit development, the mechanisms of cargospecific anterograde transport during axon extension are only starting to be explored. The most famous examples of catadromous fish is the. Yet how migration is controlled during normal development is still a largely unresolved question.
The mechanosensory lateral line system is present in all. Jun 29, 2016 delivery of proteins and organelles to the growth cone during axon extension relies on anterograde transport by kinesin motors. Lateral line system in scomberomorus niphonius teleostei. The first step in pll development is the migration of the primordium along the. Development of the posterior lateral line pll system in zebrafish involves cell migration, proliferation and differentiation of mechanosensory. Fisheries and aquaculture fisheries and aquaculture volume 1. In 1904, harrison first demonstrated that neuromasts of the posterior lateral lines of frogs rana palustris, r. At the onset of migration, the primordium of the lateral branch of the. Rostral barbels extend to anterior margin of orbit. Jul 07, 2015 movie showing in vivo imaging of lateral line migration in zebrafish. The distinctive saltwater zebra fishes pterois, used in marine aquariums, have extremely large pectoral fins, numerous extremely poisonous spines, and colourful vertical stripes.
They use it to detect depthwater pressure, prey, pretators, sense current movement and orientation in the current, as well as to avoid collisions. Hardcopies of the 4th edition of the zebrafish book can be obtained for a nominal fee from the zebrafish international resource center, 5274. The lateralline system of fish and amphibians comprises a set of discrete sensory. According to parker 1904 the first people to recognise the pores of the lateral line were stenon in 1664, lorenzini in 1678, and rivinius in 1687. The lateral line of teleost fish is usually visible externally as a row of small pores along the trunk and the head. Some species are more commonly known by the names lionfish and turkeyfish this article was most recently revised and updated by amy tikkanen, corrections manager. Zebrafish lateral line migration mbl embryology class.
Lateral line system in scomberomorus niphonius teleostei, perciformes, scombridae. Pdf direct eliminates the complexity and extra steps associated with printer drivers and middleware platforms. In zebrafish, the primordium comprises approximately 100 cells at the onset of migration. Postembryonic development of the posterior lateral line in zebrafish. May 27, 2014 in a groundbreaking study, a team of researchers that includes wwf staff discovered africas longest land mammal migration. The fish feels out distant objects by interpreting how they disrupt the intervening water. The canal organs are primarily involved in detection of lowfrequency e. Zebra has changed the game by creating pdf direct an onprinter app that allows linkos printers to directly receive and print pdfs without middleware. How to describe the function of a lateral line in a fish quora. Zebrafish danio rerio neuromast institute of molecular life sciences. Movie showing in vivo imaging of lateral line migration in zebrafish.
The lateral line system allows the detection of movement, vibration, and pressure gradients in the water surrounding an animal, providing spatial awareness and the ability to navigate in the environment. A fishs lateral line system is located in the head and runs down both sides of the body. An over view on the causes, types and role of hormones in fish migration ghulam nabi, muhammad amin, noor ullah, naveed ahmed. During its life as a parasite, each sea lamprey can kill 40 or more pounds of fish. Some particular types of migration are anadromous, in which adult fish live in the sea and migrate into fresh water to spawn, and catadromous, in which adult fish live in fresh water and migrate into salt water to spawn. The lateral line is a system of sensory organs unique to fish that allows them to identify the position of objects and movement in the surrounding water.
Migrations involve movements of the fish on a larger scale and duration than those arising during normal daily activities. This is performed by fishes like barracudas sphyraena and swordfish xiphius of the warm tropical seas. The ventral ramus of the posterior line is the familiar trunk or midbody line, formed by the posterior lateral line primordium, the migration of which is used as a staging tool kimmel et al. Zebrafish aversive taste coreceptor is expressed in both. Molecular basis of cell migration in the fish lateral line. These pores lead into an underlying canal, the lateral line canal. The lateral line seems adept at detecting the subtle movements of biological sources small prey fish, for example located some distance away.
The lateral line, also called lateral line system lls or lateral line organ llo, is a system of sense organs found in aquatic vertebrates, used to detect movement, vibration, and pressure gradients in the surrounding water. Its used to detect vibrations from preypredators, the positions of objects, and water pressure. An assay for lateral line regeneration in adult zebrafish. Zebras make africas longest land migration stories wwf. Automated screening using in vitro or cultured cell assays have yielded thousands of candidate drugs for a variety of biological targets, but these approaches have not resulted in an increase in drug discovery despite major increases in. Neuromast, primordium, stitch, migration, pattern, axis bending. Each neuromast consists of a rosette of hair cells and supporting cells, with their apical surfaces protruding through a circular hole in the. Zebrafish lateral line migration mbl embryology class of.
853 570 551 169 1442 135 6 558 1464 1055 1435 1286 139 1053 1058 1177 1223 422 436 1478 786 587 1107 426 308 878 381 1334 49 1425 1273 184 1485 837